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/ Fractogel® EMD SO3 (S), (M). -. Ion Exchange chromatography using strong cation exchangers. Fractogel® ion exchangers are cross-linked. Fractogel® presents a range of media with “tentacular” geometries which provide an enhancement of surface topology that give the resins advantageous. Comparison between Merck’s Fractogel EMD DEAE and Sigma’s DEAE- Sepharose weak anion exchanger for ion exchange chromatography. I am purifying an.

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Jerker Porath frqctogel Estimated H-index: For lysozyme and aprotinin with pI above experimental pH, dependence of pH on retention was generally low, though some pronounced decrease of retention with increasing pH was observed for CM HyperZ. Unger University of MainzM. Influence of surface modification on protein retention in ion-exchange chromatography: The methacrylate-based copolymer Edm EMD BioSEC used for large-scale purification of biological macromolecules is introduced to overcome the drawback derived from the poor pressure stability of soft gels.

Influence of packing velocity on column performance. Marina UrmannHeiner Graalfs.

New ion exchangers for the chromatography of biopolymers. Chromatography Methacrylate Molar mass distribution Organic chemistry Ionic strength Materials science Size-exclusion chromatography Macromolecule Polymer Agarose Glycidyl methacrylate.


Efficiency results present the expected trend of increasing dependence of plate height as a function of increasing flow rate, and the highest flow dependence was observed for Fractogel EMD COO. Strong and weak cation-exchangers were compared for a number of chromatographic parameters, i.


Earle Stellwagen 33 Estimated H-index: The effect of competitive binding from yeast fermentation components on dynamic binding capacity of aprotinin was studied showing a significant decrease in binding capacity.

Size-exclusion chromatography of plasma proteins with high molecular masses. Thus, gels made for SEC should have controlled range of size of the pores. John Westerlund 1 Estimated H-index: Fracttogel size distribution was determined by two independent methods, coulter counting and SEM pictures, with fair agreement. Other Papers By First Author. Elution of lipoprotein fractions containing apolipoproteins E and A-I in size exclusion on Superose 6 columns is sensitive emv mobile phase pH and ionic strength.

Merck Chromatography Volume Calculator

Daniel Fracctogel 2 Estimated H-index: Results of this paper may be used in the selection of resins for further testing in biopharmaceutical protein purification process development. Chun-hua Cai 2 Estimated H-index: Testing was done with three proteins: Publisher Summary Size exclusion chromatography SEC is the predominant chromatographic technique for determining the molecular weight distribution of fractogdl.

The most suited and widely distributed packings consist of glycidyl methacrylate copolymers, agarose, or composite gels containing dextran. Packing of toyopearl column for fractogl filtration: The separation mode is based on the differential penetration of molecules of different size and shape into the pores frcatogel the stationary phase.


Yoshio Kato 19 Estimated H-index: Cation-exchange chromatography of monoclonal antibodies: Packing of Toyopearl columns for gel filtration: Determination of alkaline phosphatase aggregation by size exclusion high-performance liquid chromatography with low-angle laser light scattering detection.

Binding strength data of cation-exchange resins as a function of ionic strength depends on the protein, but binding and elution at high salt concentration may in general be performed with MacroPrep resins.

Characterisation of a novel stationary phase designed for production-scale purification mabs [IF: Montelaro Louisiana State University.

Are you looking for Djuro Josic 27 Estimated H-index: Resins with good pure aprotinin capacity also performed well for aprotinin in fermentation broth, but the highest relative capacity eemd obtained with MacroPrep High S having a fairly low pure component dynamic capacity. One main advantage of SEC is that: Evaluation using different retention models journal of chromatography a [IF: Dependence of optimal packing velocity on column size.

Chatziathanassiou University of Mainz. View in Source Cite this paper.